Bacterial disease resistance in Arabidopsis through flagellin perception. Article ( PDF Available) in Nature () · May with. Bacterial disease resistance in Arabidopsis through flagellin perception. PUBLISHED: Apr CITE AS: Nature. Apr 15; () In Arabidopsis thaliana, the receptor kinase flagellin sensing 2 (FLS2) confers recognition of bacterial flagellin (flg22) and activates a manifold defense response. In plants that are resistant to these successful pathogens, plant PAMP perception system, whose components are often shared across.

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Even though simulations have shown that compound tests, which combine the properties of summary statistics, are not easily confounded by demographic effects Zeng et al.

Geographic variation in adaptation at the molecular level: This flg22 peptide has the highest binding affinity known to date and elicits the strongest responses in A. Differences in FLS2 transcript abundance did not reflect variation in protein abundance, so it is not clear whether cis -regulatory differences underpin the QTL.

Natural variation for flginduced inhibition of seedling growth was measured in 37 of the 45 genotypes reported in figure 1. Recommendations Abstract Comments You have reached your article limit. We could therefore document the existence of genetic variation located in the cis -regulatory region of the FLS2 locus. RNA was extracted from each of 10—12 independent seedlings in two biological independent trials. Variation in the repertoire of plant R genes is also remarkable; individuals within species generally display differences in their resistance profiles Laine et al.

This is potentially due to players in the pathway other than BAK1 such as, for example, the signaling mitogen-activated MAP kinases 3, perceptiin, and 6 Boller and Felix Heritability was estimated for seedlings fresh weight in absence and presence of flg You expect to receive, or in the past 4 years have received, shared grant support or other funding with any of arabidopsie authors.

Bacterial disease resistance in Arabidopsis through flagellin perception.

In the future, identifying the evolutionary forces driving variation in plant flagellin perception promises to find resonance beyond the borders of plant science. We analyzed nucleotide variation segregating at FLS2 in A. The correlation between flg and elfelicited inhibition of seedling growth points to shared components controlling FLS2 and EFR receptor abundance, which could affect other PAMP perception systems and thus be key regulators of PAMP-triggered immunity.


This long-term accumulative response likely employs many molecular components of the flgsignaling pathway. The genotype Ws-0 is known to carry a non-functional FLS2 allele and was used as a negative control Zipfel et al.

The observed variation correlates with both the severity of elicited defense responses and bacterial proliferation.

Alternatively, FLS2 could evolve so that its affinity for flagellin tracks the flagellin alleles with which it interacts. A focus on R gene recognition has dominated early studies of the molecular evolution of immunity genes in plants Stahl et al. We established experimental probabilities for the compound test DHEW as the proportion of loci with comparable combinations of outlier DHnand EW values e.

Pleiotropy modulates the efficacy of selection in Drosophila melanogaster.

Resistane studied functional variation mediated by FLS2 in A. Recommend to your librarian. Analysis of flagellin perception mediated by flg22 receptor OsFLS2 in rice.

A summary of the content will be automatically included. Although fls2 mutant plants are as susceptible as the wild type when bacteria are infiltrated into leaves, they are more susceptible to the pathogen Pseudomonas syringae pv.

Immunoblot analysis of whole protein extracts was conducted to compare FLS2 protein abundance within groups of A. Molecular population genetics and the search for adaptive evolution in bactefial.

Bacterial disease resistance in Arabidopsis through flagellin perception.

Among distantly related species, it is known that the specificity of ligand recognition can change Chinchilla et al. Pyrosequencing quantification of relative allelic expression was performed on cDNA samples. To date, no other genotype has been found to harbor this mutation. In our study, variation in flg22 binding was associated with the severity of bacterial infection. Within and among close relatives, PAMP perception evolves quantitatively, which contrasts with the changes in recognition typically associated with the evolution of R genes.

However, our study also shows that evolution at the receptor locus alone does not explain the evolution of flagellin perception; instead, components common to pathways downstream of PAMP perception likely contribute to the observed quantitative variation.

Genome-wide association arabidopssi of phenotypes in a common set of Arabidopsis thaliana inbred lines.


We considered population structure parameters when correlating quantitative flgbinding and flginduced inhibition of seedling growth. F reserves the right to remove any comments that it considers in its absolute discretion to be inappropriate, offensive or otherwise in breach of the Terms and Conditions relating to Materials including Comments.

Molecular identification and characterization of the tomato flagellin receptor LeFLS2, an orthologue of Arabidopsis Arbaidopsis exhibiting characteristically different perception specificities.

Resistance variation within and among host populations in a plant—pathogen metapopulation: Yet, recombination events were detected, and the breakpoints, that is, positions where neighboring SNPs show four gametic types, revealed a sequence fragment with a distinct frequency spectrum of polymorphism. These data altogether show that the intensity of flg22 binding correlates positively with the severity of one of the responses triggered after FLS2 activation.

Pattern recognition receptors require N-glycosylation to mediate plant immunity. The presence of FLS2 receptors in distant taxa, despite evasion of flagellin recognition by some microbial strains, suggests that FLS2 retains its relevance for defense Felix et al.

The flginduced inhibition of seedling growth depends not only on FLS2 but also on BAK1 and other unknown molecular components. For example, flagellin, or its active surrogate the peptide flg22, is perceived by the receptor flagellin sensing 2 FLS2 in plants from diverse families, such as Arabidopsis, tomato, and rice Zipfel et al. Dynamics of disease resistance polymorphism at the Rpm1 locus of Arabidopsis.

Bacterial disease resistance in Arabidopsis through flagellin perception. – FPrime

Here we show that treatment of plants with flg22, a peptide representing the elicitor-active epitope of flagellin, induces the expression of numerous defence-related genes and triggers resistance to pathogenic bacteria in wild-type plants, but not in plants carrying mutations in the flagellin receptor gene FLS2. Neither of the above. Yet, no excess of nonsynonymous mutations is observed, as has been shown for plant R genes suspected to tightly coevolve with their ligand Bergelson et al.